#Leica acquire download 3.3 software#
#Leica acquire download 3.3 windows#

#Leica acquire download 3.3 software#

Fluoview software saves images in a non-proprietary format to facilitate the use of the raw data files in 3rd party image analysis or visualization software such as ImageJ, MetaMorph, AutoQuant, Volocity and Imaris. brightness vs time) confocal images obtained in a z-series can be volume rendered and analyzed in 3 dimensions. Integral Fluoview software allows for analysis of saved images in 2 dimensions (e.g. Images are saved to local secondary 250 GB hard drive and then transferred to a 2 TB mirrored RAID-1 network-attached storage (NAS) device that is accessible from core workstations or individual laboratory computers through the UM medical campus 4 Gbps network. The system is also equipped with Differential Interference Contrast (DIC) objectives and condensers and has the ability to capture transmitted light images with a highly sensitive photomultiplier (PMT) transmission detector. Optical sections in the z plane can be collected using a stop motor attached to the fine focus control of the microscope and driven by Fluoview software. The FV500’s acoustical optical tuning filter (AOTF) and adjustable scan speeds provides for minimal specimen fading, sequential scanning for reduced fluorescence cross talk, multiple regions of excitation, high resolution imaging (up to 2048 x 2048 pixels) of fixed or static samples, and rapid recording of kinetic events. Users are able to image a wide variety of fluorophores with laser excitation that includes Blue Violet (405nm) Multi-Line Argon Blue (458,488,515nm), Helium Neon Green (543nm) and Helium Neon Red (633nm) for standard Blue, Green, Red and Far-Red fluorochromes.

#Leica acquire download 3.3 windows#

The FV500 is controlled by a 2.4 Ghz personal computer under Windows XP and is capable of imaging 5 separate channels simultaneously (4 fluorescence + 1 transmitted light photomultiplier detectors) offering highly efficient, maximum emission sensitivity and the ability to record scanned images in 12 bits or 4096 gray levels, thus allowing quantitative linear measurement of fluorescence within regions of low contrast as well as very high contrast. The results of experiments verify the feasibility, effectiveness and accuracy of the proposed method.Olympus FluoView 500 Laser Scanning Confocal Microscope LSCM The uncertainty sources that affect the accuracy of the proposed method are analyzed and the main observation procedures for practical use are provided. And the height difference between them can be calculated based on the principle of trigonometric levelling. Carrying a 360° prism as the target and hovering in the air, the UAV can be sighted by both calibrated and synchronized robotic total stations near two benchmarks to conduct synchronous observations with the help of the automatic target recognition (ATR) and tracking systems.

Therefore, this paper proposes the combination of unmanned aerial vehicles (UAVs) and robotic total stations in determining height differences across obstacles. When benchmarks are blocked by obstacles such as buildings, woods, hills, etc., it is time- and energy-consuming to acquire the height differences between them through classical geometric levelling because of the detour.